A collection of powerful new techniques for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation. The applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene expression.
| Author: Piet Herdewijn |
| Publisher: Humana |
| Publication Date: Sep 01, 2004 |
| Number of Pages: 436 pages |
| Binding: Hardback or Cased Book |
| ISBN-10: 1588292339 |
| ISBN-13: 9781588292339 |
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