Neural Stem Cells: Methods and Protocols

Although there has been an explosion of interest and technology in the study of neural stem cells, many questions related to stem cell properties and neural stem cell lineage and differentiation still linger. This second edition revises and expands upon the successful first edition in order to provide the most current, cutting-edge methods of today for the scientists working to answer these questions. The use of these step-by-step, readily reproducible laboratory protocols will allow investigators to produce pure populations that can serve as a means of understanding the biology of neural stem cells and adapting them for transplantation into disease models. Following the Methods in Molecular Biology(TM) series format, each chapter has an extended introduction for background, a list of necessary materials, and tips on troubleshooting and avoiding known pitfalls. This is an excellent source of information and inspiration for scientists longing to use these powerful cells against neurogenetic and neurodegenerative diseases.