This manual introduces the reader to bacic methods used in the isolation, cloning and analysis of genetic material. The protocols include RT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific mutagenesis, analysis of protein DNA-specific interaction, cell-free protein synthesis and product electrophoretic and immunological analysis.
| Author: Valdis Berzins |
| Publisher: Springer |
| Publication Date: Jul 07, 2012 |
| Number of Pages: 169 pages |
| Binding: Paperback or Softback |
| ISBN-10: 364248977X |
| ISBN-13: 9783642489778 |