Effect of cryoprotectant dilution on equine semen

The aim of this study was to evaluate in vitro motility and viability parameters when diluting the 5% post-thaw cryoprotectant dimethylformamide to concentrations of 2.5 and 1.25%, using two commercial diluents added to cryopreserved equine semen. After thawing, the samples were diluted in order to maintain the final concentrations (2.5 and 1.25%) of cryoprotectant, using two commercial diluents (FR4(R) and Botu-Crio(R)) at two times: initial (Ti) and final (Tf). Thirteen different ejaculate samples from five national breed stallions were used. Motility parameters were observed using computerized analysis and plasma membrane integrity using epifluorescence microscopy. There was an improvement in total and progressive sperm motility parameters at the final time (P0.05, between the treatments in terms of plasma membrane integrity.

Author: Marina Zimmermann

Publisher: Our Knowledge Publishing

Publication Date: Oct 28, 2024

Number of Pages: 52 pages

Binding: Paperback or Softback

ISBN-10: 6208240840

ISBN-13: 9786208240844