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Molecular Biology Techniques: Polymerase Chain Reaction (PCR)
Product Code:
9786208515492
ISBN13:
9786208515492
Condition:
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$38.61
Molecular Biology Techniques: Polymerase Chain Reaction (PCR)
$38.61
The polymerase chain reaction (PCR) has as its main application the possibility of amplifying a segment of DNA, producing millions of copies of the selected segment. Gene cloning aims to introduce a selected gene into bacteria that are identical to each other, and this technique requires the presence of a cloning vector capable of amplifying a fragment of DNA cloned into them. The E gene of the dengue virus (DENV) is the most widely used for molecular studies due to the importance of the E protein. The aim of this study is to establish techniques for amplifying the E gene of DENV-2 and DENV-4. The DNA molecule was obtained from FIOCRUZ in Recife; the primers were selected in such a way as to encompass the E gene. PCR amplification was satisfactorily established for the E gene. Once determined, these techniques are essential in the stages of obtaining recombinant DNA in the process of cloning and expressing the dengue E protein.
| Author: Beatriz Dantas Guimarães |
| Publisher: Our Knowledge Publishing |
| Publication Date: Dec 30, 2024 |
| Number of Pages: 52 pages |
| Binding: Paperback or Softback |
| ISBN-10: 6208515491 |
| ISBN-13: 9786208515492 |
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